文章摘要
通过理性改造提高11家族木聚糖酶热稳定性研究进展
Advances in Improving Xylanase Family 11 Thermostability by Rational Reconstruction
  
DOI:doi:10.3969/j.issn.1005-7021.2017.05.018
中文关键词: 11家族木聚糖酶  热稳定性  二硫键  糖基化  疏水作用
英文关键词: family 11 xylanase  thermostability  disulfide bond  glycosylation  hydrophobic effect
基金项目:国家海洋局海洋生物活性物质与现代分析技术重点实验室开放基金项目(MBSMAT-2015-01)
作者单位
吴占东 1.青岛大学 环境科学与工程学院山东 青岛 2660712.滨州职业学院 继续教育学院,山东 滨州 256603 
韩文杰 青岛大学 环境科学与工程学院山东 青岛 266071 
周同 青岛大学 环境科学与工程学院山东 青岛 266071 
张培玉 青岛大学 环境科学与工程学院山东 青岛 266071 
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中文摘要:
      木聚糖是植物细胞中含量最高的半纤维素,是一种重要的微生物资源,也是自然界中含量丰富的多糖之一 。木聚糖酶是能够将复杂的木聚糖结构水解为木寡糖的一类酶系,按其结构域可分为不同的家族,其中大部分木聚糖酶属于糖苷水解酶的10和11家族,而11家族的木聚糖酶由于分子量较小,比酶活较高等诸多优点使其在饲料、造纸、食品、能源工业有着广阔的应用前景。但是商业化木聚糖酶必须易于生产,酶活较高,并且能够适应工业过程中的各种严格条件,例如饲料与造纸工业过程中有时需要瞬时提高反应温度,这就要求参与这一系列反应的木聚糖酶在高温下有着较高的比酶活和热稳定性。但是一般条件下,很多微生物分泌的木聚糖酶达不到此要求,因此,通过合理的分子生物学方法提高木聚糖酶的热稳定性使其在工业生产中发挥更大的作用是人们不断追求的目标。 本文重点阐述二硫键、糖基化、疏水作用等蛋白的重要性质, 结合作者自身实验结果,讨论了这些性质对11家族木聚糖酶热稳定性的影响。为进一步加深木聚糖酶作用机制的了解、指导木聚糖酶的分子改良有提供参考。
英文摘要:
      Xylan is the highest content of hemicellulose in plant cells and important microbial resources, it is also one of abundant content of polysaccharides in Nature. Xylanase contains a series of enzymes that can hydrolyze xylan into xylo-oligosaccharides, according to the domain can be divided into different families, of which most of the wooden xylanase belongs to glycoside hydrolase family 10 and 11; And xylanase of family 11 have draw much attention due to the smaller molecular weight, high specific enzyme activity and many other advantages and make it possessing a broad application prospect in the forage, papermaking, food, energy industries. However, the commercialization of xylanase must be easy to produce, have higher enzyme activity, and suitable to the strict industrial processes in various conditions, such as in forage and papermaking industrial processes, sometimes need instantaneous increasing reaction temperature, and these require xylanase to have fairly high specific activity and thermostability under high temperature. But in ordinary conditions, many microorganisms secrete xylanase can not meet this requirement. Therefore, improving the thermostability of xylanase by some reasonable molecular biology methods is people′s constant pursuit of the goal. This paper focuses on explanation of the important properties of the protein, such as the disulfide bond, glycosylation and hydrophobic interaction, and expaciate the effect of these factors on xylanase thermostability of family 11 combined with the author′s personal experiment results. It will have great significance to further deepen understand the mechanism of xylanase and direct the molecular modification of xylanase.
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