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| 内生真菌与大花红景天互作的RAPD标记分析 |
| RAPD Marker Analysis of Rhodiola crenulata Interacted with Endophytic Fungi ZPRs-R-11 |
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| DOI：doi:10.3969/j.issn.1005-7021.2017.05.002 |
| 中文关键词: 红景天 内生真菌 RAPD 遗传性状 聚类分析 |
| 英文关键词: Rhodiola spp. endophytic fungi RAPD genetic character cluster analysis |
| 基金项目:国家自然科学基金项目(31270383,31670328)；山西省自然科学基金项目(2014011029-1) |
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| 中文摘要: |
| 首次采用RAPD分子标记法研究内生真菌接种宿主红景天条件下，前者对后者DNA序列水平遗传性状的影响。结果显示，12条RAPD引物对ZPRs-R-11接种的红景天扩增出87条条带，多态性百分比为83.91%。聚类分析结果表明，内生真菌ZPRs-R-11接种的红景天可分为三个遗传分支，共培养时间越长，组培苗与内生真菌之间的遗传相似系数越大；红景天接种后，其基因流Nm为0.209 8。研究结果为内生真菌与宿主大花红景天共生条件下内生真菌对大花红景天的品质改良和研究提供参考。 |
| 英文摘要: |
| RAPD molecular marker analysis was adopted firstly to investigate the effects of genetic character of host Rhodiola crenulata inoculated with endophytic fungus ZPRs-R-11. The results showed that a total of 87 bands were amplified by 12 RAPD primers, and the percentage of polymorphism was 83.91% in R. crenulata inoculated with ZPRs-R-11. Cluster analysis indicated that R. crenulata plants inoculated with ZPRs-R-11 was clustered into three genetic branches. The similarity coefficient was higher when co-cultivation between endophytic fungus and plant got more long time, and the genetic similarity coefficient between tissue culture seedling and endophytic fungi got larger. The gene flows were at Nm=0.208 9 after R. crenulata was inoculated. The results of the study provided scientific foundation to improve the quality of R. crenulata by endophytic fungus and further study on R. crenualata under symbiotic condition of R. crenulata with endophytic fungus. |
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