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| 低温纤维素降解菌的筛选及其酶学性质初步研究 |
| Screening of Cryophilic Cellulose-Degradable Bacterium and Its Zymological Properties |
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| DOI： |
| 中文关键词: 低温纤维素酶 细菌 筛选 酶学性质 |
| 英文关键词: cryo-cellulase bacteria screening zymological properties |
| 基金项目:河南省科技攻关项目，河南省重点科技攻关项目 |
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| 摘要点击次数: 112 |
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| 中文摘要: |
| 从青藏高原冰川雪样恢复出的4株细菌中筛选出1株降解纤维素能力比较高的菌株LHG-C-9.经16S rDNA序列分析,初步鉴定为假单胞菌属.对该菌所产纤维素酶的性质进行了初步研究,其最适作用温度为 30 ℃;对热敏感;最适pH 8.0;属碱性温酶.该低温纤维素酶在纺织、造纸、环保、医药和饲料等行业可望有广泛的应用前景. |
| 英文摘要: |
| A strain LHG-C-9 with high cellulose degradation ability was screened from four bacterial strains recovered from snow samples collected in Qinghai-Tibet plateau glacier. Initial identification indicated that it belonged to Pseudomonas sp. through its 16S rDNA sequence analysis. The initial study on its properties showed that the most suitable temperature of the cellulase was 30 ℃; Sensitive to heat, the most suitable pH value was 8.0, and belonged to alkaline cryo-enzyme. It is hopeful and has wide application vista in textile, paper making, environment protection, medicine, forage, and other industries. |
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